|
KMID : 0385219980080020001
|
|
Korean Journal of Gerontology
1998 Volume.8 No. 2 p.1 ~ p.7
|
|
|
Immunohistochemical Analysis of Nitric Oxide Synthase-positive Cells in Autoimmune Encephalomyelitis in Rats
|
|
Jin Jae-Kwang
Moon Chang-Jong
Wie Myung-Bok
Jeong Kyu-Shik
Park Sang-Jun
Shin Tae-Kyun
|
|
|
Abstract
|
|
|
|
To elucidate the roles of neuronal nitric oxide synthase(nNOS) and inducible NOS(iNOS) in the central nervous system in experimental autoimmune encephalomyelitis(EAE), we examined the distribution of apoptotic cells and analyzed nNOS-positive(nNOS+) and iNOS-positive (iNOS+) cells in spinal cords with EAE. In EAE lesions, apoptotic cells were evenly distributed in the spinal cord parenchyma, as well as in perivascular inflammatory lesions. Some apoptotic cells had intimate structural relationships with neurons and glial cells. Immunohistochemistry showed that nNOS+ cells, including neurons, increased in number in the peak stage of EAE. The increased immunoreactivity of nNOS in neurons and astrocytes persisted during the EAE recovery stage. In addition, the number of iNOS+ cells increased in perivascular lesions. Some astrocytes distant from EAE lesions also showed iNOS immunoreactivity. These findings suggest that nitric oxide(NO) is generated in the spinal cords in EAE via either nNOS or iNOS. The structural relationship between apoptosis and NOS-positive cells in EAE implies that NO may play an important role in eliminating inflammatory cells via apoptosis, which results in the spontaneous recovery from paralysis in EAE.
|
|
|
KEYWORD
|
|
|
Neuronal nitric oxide synthase, Experimental autoimmune encephalomyelitis, Apoptosis
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|
|